Draft genome sequence of a clinical Acinetobacter baumannii isolate of new sequence type ST1688 from Saudi Arabia

Muhammad Yasir; Muhammad Waseem Shah; Asif A Jiman-Fatani; Saad B Almasaudi; Hussain Ahmad; Maha Alawi; Esam I Azhar

doi:10.1016/j.jgar.2019.06.021

Draft genome sequence of a clinical Acinetobacter baumannii isolate of new sequence type ST1688 from Saudi Arabia

J Glob Antimicrob Resist. 2019 Sep:18:151-152. doi: 10.1016/j.jgar.2019.06.021. Epub 2019 Jul 8.

Authors

Muhammad Yasir¹, Muhammad Waseem Shah², Asif A Jiman-Fatani³, Saad B Almasaudi⁴, Hussain Ahmad⁵, Maha Alawi⁶, Esam I Azhar⁷

Affiliations

¹ Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia; Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia. Electronic address: yasirkhattak.mrl@gmail.com.
² Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia; Biology Department, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
³ Department of Medical Microbiology and Parasitology, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia; Clinical and Molecular Microbiology Laboratories, King Abdulaziz University Hospital, Jeddah, Saudi Arabia.
⁴ Biology Department, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
⁵ Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia.
⁶ Department of Medical Microbiology and Parasitology, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia; Infection Control & Environmental Health Unit, King Abdulaziz University Hospital, King Abdulaziz University, Jeddah, Saudi Arabia.
⁷ Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia; Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.

PMID: 31295580
DOI: 10.1016/j.jgar.2019.06.021

Abstract

Objectives: Acinetobacter baumannii has emerged as a prevalent multidrug-resistant nosocomial pathogen worldwide. Here we report the draft genome sequence of A. baumannii strain Ab174 isolated from a neonatal patient diagnosed with acute peritonitis.

Methods: The draft genome sequence of A. baumannii Ab174 was determined using a MiSeq platform (Illumina Inc., San Diego, CA) using v.3, 2×30-bp chemistry. Genomic assembly was performed using SPAdes 3.9 algorithm.

Results: The draft genome of A. baumannii Ab174 is 3 747 065bp in length and was classified as a new sequence type (ST1688). The genome of A. baumannii Ab174 has a G+C content of 39% and harbours two plasmids. The antimicrobial resistance gene bla_ADC-25 and the virulence factor gene for penicillin-binding protein G (pbpG) as well as 17 genomic islands and 14 insertion sequences were identified in the genome of A. baumannii Ab174.

Conclusion: The genome sequence of A. baumannii strain Ab174 can be used as a reference sequence for the new ST1688. These data will facilitate further understanding of genomic variation in isolates from different geographical regions.

Keywords: Acinetobacter baumannii; Genome sequence; ST1688; Saudi Arabia.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acinetobacter baumannii / drug effects
Acinetobacter baumannii / genetics*
Acinetobacter baumannii / isolation & purification*
Anti-Bacterial Agents / pharmacology
Base Composition
Base Sequence
Drug Resistance, Multiple, Bacterial / genetics*
Genes, Bacterial / genetics*
Genetic Variation
Genome, Bacterial / genetics*
Humans
Microbial Sensitivity Tests
Peritonitis / microbiology
Plasmids
Saudi Arabia
Virulence Factors

Substances

Anti-Bacterial Agents
Virulence Factors