Comparing methods of genetic manipulation in Bacillus subtilis for expression of recombinant enzyme: Replicative or integrative (CRISPR-Cas9) plasmid?

J Microbiol Methods. 2019 Sep:164:105667. doi: 10.1016/j.mimet.2019.105667. Epub 2019 Jul 8.

Abstract

The present study evaluated the stability of Bacillus subtilis strains transformed with a replicative or integrative plasmid (via CRISPR-Cas9) to express a recombinant phytase. Both transformation methods did not affect the growth of B. subtilis, but the stability of the construct and the enzymatic activity was reduced in the strain transformed with the replicative plasmid.

Keywords: Bacterial transformation; CRISPR-Cas9; Phytase; Plasmid stability.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 6-Phytase / genetics
  • Bacillus subtilis / genetics*
  • Bacterial Proteins / genetics*
  • CRISPR-Cas Systems*
  • Clustered Regularly Interspaced Short Palindromic Repeats
  • DNA Replication
  • Gene Expression Regulation, Bacterial
  • Genes, Bacterial / genetics
  • Genetic Techniques*
  • Plasmids*
  • Recombinant Proteins*
  • Transformation, Bacterial

Substances

  • Bacterial Proteins
  • Recombinant Proteins
  • 6-Phytase