Real-time OCT guidance and multimodal imaging monitoring of subretinal injection induced choroidal neovascularization in rabbit eyes

Yanxiu Li; Wei Zhang; Van Phuc Nguyen; Rachel Rosen; Xueding Wang; Xiaobo Xia; Yannis M Paulus

doi:10.1016/j.exer.2019.107714

Real-time OCT guidance and multimodal imaging monitoring of subretinal injection induced choroidal neovascularization in rabbit eyes

Exp Eye Res. 2019 Sep:186:107714. doi: 10.1016/j.exer.2019.107714. Epub 2019 Jul 6.

Authors

Yanxiu Li¹, Wei Zhang², Van Phuc Nguyen³, Rachel Rosen⁴, Xueding Wang⁵, Xiaobo Xia⁶, Yannis M Paulus⁷

Affiliations

¹ Department of Ophthalmology, Xiangya Hospital, Central South University, NO. 87 Xiangya Road, Kaifu District, Changsha, Hunan, 410008, PR China; Department of Ophthalmology and Visual Sciences, University of Michigan, 1000 Wall Street, Ann Arbor, MI, 48105, United States. Electronic address: yanxiuli@umich.edu.
² Department of Biomedical Engineering, University of Michigan, 2200 Bonisteel Blvd, Ann Arbor, MI, 48109, United States. Electronic address: zhawei@umich.edu.
³ Department of Ophthalmology and Visual Sciences, University of Michigan, 1000 Wall Street, Ann Arbor, MI, 48105, United States. Electronic address: vanphucn@umich.edu.
⁴ Department of Ophthalmology and Visual Sciences, University of Michigan, 1000 Wall Street, Ann Arbor, MI, 48105, United States. Electronic address: rarosen@umich.edu.
⁵ Department of Biomedical Engineering, University of Michigan, 2200 Bonisteel Blvd, Ann Arbor, MI, 48109, United States. Electronic address: xdwang@umich.edu.
⁶ Department of Ophthalmology, Xiangya Hospital, Central South University, NO. 87 Xiangya Road, Kaifu District, Changsha, Hunan, 410008, PR China. Electronic address: xbxia21@163.com.
⁷ Department of Ophthalmology and Visual Sciences, University of Michigan, 1000 Wall Street, Ann Arbor, MI, 48105, United States; Department of Biomedical Engineering, University of Michigan, 2200 Bonisteel Blvd, Ann Arbor, MI, 48109, United States. Electronic address: ypaulus@med.umich.edu.

Abstract

Choroidal neovascularization (CNV) is a major cause of vision loss that consists of abnormal growth of new blood vessels from the choroidal vasculature. High resolution in vivo imaging of animal models is essential to better elucidate and conduct research on CNV. This study evaluates a novel multimodal imaging platform combining optical coherence tomography (OCT) and photoacoustic microscopy (PAM). Using real-time OCT guidance subretinal injection to induce and multimodality imaging system to monitor CNV over time in rabbit eyes. The significance of our work lies in providing the optimal setting and conditions to make use of the OCT image guided system to improve the consistency and reproducibility of experimental results in subretinal injection induced CNV model in rabbits. For the first time, this study successfully demonstrated the dual-modality PAM-OCT system, without using exogenous contrast agents, can detect and visualize CNV in the rabbit eye with high resolution. This is promising system for diagnosing and monitoring CNV.

Keywords: Choroidal neovascularization; Intraoperative OCT; OCT; Optical coherence tomography; Photoacoustic microscopy; Real-time imaging; Subretinal injection.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Choroidal Neovascularization / diagnostic imaging*
Choroidal Neovascularization / etiology
Diagnostic Techniques, Ophthalmological*
Injections, Intraocular / adverse effects
Microscopy, Acoustic / methods*
Multimodal Imaging
Rabbits
Reproducibility of Results
Tomography, Optical Coherence / methods*

Abstract

Publication types

MeSH terms

Grants and funding