Chemoselective probe for detailed analysis of ketones and aldehydes produced by gut microbiota in human samples

Louis P Conway; Neeraj Garg; Weifeng Lin; Miroslav Vujasinovic; J-Matthias Löhr; Daniel Globisch

doi:10.1039/c9cc04605d

Chemoselective probe for detailed analysis of ketones and aldehydes produced by gut microbiota in human samples

Chem Commun (Camb). 2019 Aug 11;55(62):9080-9083. doi: 10.1039/c9cc04605d. Epub 2019 Jul 9.

Authors

Louis P Conway¹, Neeraj Garg¹, Weifeng Lin¹, Miroslav Vujasinovic², J-Matthias Löhr³, Daniel Globisch¹

Affiliations

¹ Department of Medicinal Chemistry, Science for Life Laboratory, Uppsala University, Box 574, SE-75123 Uppsala, Sweden. Daniel.globisch@scilifelab.uu.se.
² Department for Digestive Diseases, Karolinska University Hospital, Stockholm, Sweden.
³ Department for Digestive Diseases, Karolinska University Hospital, Stockholm, Sweden and Department of Clinical Science, Intervention and Technology (CLINTEC), Karolinska Institute, Stockholm, Sweden.

PMID: 31287110
DOI: 10.1039/c9cc04605d

Abstract

New strategies are required for the discovery of unknown bioactive molecules produced by gut microbiota in the human host. Herein, we utilize a chemoselective probe immobilized to magnetic beads for analysis of carbonyls in human fecal samples. We identified 112 metabolites due to femtomole analysis and an increased mass spectrometric sensitivity by up to six orders of magnitude.

MeSH terms

Aldehydes / analysis*
Aldehydes / metabolism
Feces / chemistry*
Feces / microbiology
Female
Gastrointestinal Microbiome / physiology*
Humans
Ketones / analysis*
Ketones / metabolism
Male
Molecular Probes / analysis*
Molecular Probes / chemical synthesis
Molecular Probes / chemistry*
Molecular Structure
Particle Size
Surface Properties

Substances

Aldehydes
Ketones
Molecular Probes