Rapid MALDI mass spectrometry imaging for surgical pathology

Sankha S Basu; Michael S Regan; Elizabeth C Randall; Walid M Abdelmoula; Amanda R Clark; Begoña Gimenez-Cassina Lopez; Dale S Cornett; Andreas Haase; Sandro Santagata; Nathalie Y R Agar

doi:10.1038/s41698-019-0089-y

Rapid MALDI mass spectrometry imaging for surgical pathology

NPJ Precis Oncol. 2019 Jul 4:3:17. doi: 10.1038/s41698-019-0089-y. eCollection 2019.

Authors

Affiliations

¹ 1Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115 USA.
² 2Department of Neurosurgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115 USA.
³ 3Department of Radiology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115 USA.
⁴ Bruker Daltonics, Billerica, MA 01821 USA.
⁵ 5Bruker Daltonik GmbH, Bremen, Germany.
⁶ 6Ludwig Center at Harvard, Harvard Medical School, Boston, MA 02115 USA.

^# Contributed equally.

Abstract

Matrix assisted laser desorption ionization mass spectrometry imaging (MALDI MSI) is an emerging analytical technique, which generates spatially resolved proteomic and metabolomic images from tissue specimens. Conventional MALDI MSI processing and data acquisition can take over 30 min, limiting its clinical utility for intraoperative diagnostics. We present a rapid MALDI MSI method, completed under 5 min, including sample preparation and analysis, providing a workflow compatible with the clinical frozen section procedure.

Keywords: Metabolomics; Molecular imaging; Molecular medicine; Surgical oncology.

Abstract

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