Abstract
An adenylate cyclase activity in Medicago sativa L. (alfalfa) roots was partially characterized. The enzyme activity remains in the supernatant fluid after centrifugation at 105,000 g and shows in crude extracts an apparent Mr of about 84,000. The enzyme is active with Mg2+ and Ca2+ as bivalent cations, and is inhibited by EGTA and by chlorpromazine. Calmodulin from bovine brain or spinach leaves activates this adenylate cyclase.
MeSH terms
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Adenylyl Cyclase Inhibitors
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Adenylyl Cyclases / metabolism*
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Calcium / pharmacology
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Calmodulin / pharmacology
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Centrifugation, Density Gradient
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Chlorpromazine / pharmacology
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Chromatography, DEAE-Cellulose
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Chromatography, Gel
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Egtazic Acid / pharmacology
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Fluphenazine / pharmacology
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Medicago sativa / enzymology*
Substances
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Adenylyl Cyclase Inhibitors
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Calmodulin
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Egtazic Acid
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Adenylyl Cyclases
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Fluphenazine
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Calcium
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Chlorpromazine