A nano-sized gel-in-oil suspension for transcutaneous protein delivery

Safrina Dyah Hardiningtyas; Seiya Nagao; Emiko Yamamoto; Nana Shirakigawa; Rie Wakabayashi; Masahiro Goto; Hiroyuki Ijima; Noriho Kamiya

doi:10.1016/j.ijpharm.2019.118495

A nano-sized gel-in-oil suspension for transcutaneous protein delivery

Int J Pharm. 2019 Aug 15:567:118495. doi: 10.1016/j.ijpharm.2019.118495. Epub 2019 Jul 2.

Authors

Safrina Dyah Hardiningtyas¹, Seiya Nagao², Emiko Yamamoto³, Nana Shirakigawa⁴, Rie Wakabayashi⁵, Masahiro Goto⁶, Hiroyuki Ijima⁷, Noriho Kamiya⁸

Affiliations

¹ Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, Motooka 744, Nishi-ku, Fukuoka 819-0395, Japan. Electronic address: safrina_dyah@apps.ipb.ac.id.
² Department of Chemical Engineering, Graduate School of Engineering, Kyushu University, Motooka 744, Nishi-ku, Fukuoka 819-0395, Japan. Electronic address: s_nagao@kyudai.jp.
³ Department of Chemical Engineering, Graduate School of Engineering, Kyushu University, Motooka 744, Nishi-ku, Fukuoka 819-0395, Japan. Electronic address: yamaemi@kyudai.jp.
⁴ Department of Chemical Engineering, Graduate School of Engineering, Kyushu University, Motooka 744, Nishi-ku, Fukuoka 819-0395, Japan. Electronic address: shirakigawa@chem-eng.kyushu-u.ac.jp.
⁵ Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, Motooka 744, Nishi-ku, Fukuoka 819-0395, Japan. Electronic address: rie_wakaba@mail.cstm.kyushu-u.ac.jp.
⁶ Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, Motooka 744, Nishi-ku, Fukuoka 819-0395, Japan; Division of Biotechnology, Center for Future Chemistry, Kyushu University, Japan. Electronic address: m-goto@mail.cstm.kyushu-u.ac.jp.
⁷ Department of Chemical Engineering, Graduate School of Engineering, Kyushu University, Motooka 744, Nishi-ku, Fukuoka 819-0395, Japan. Electronic address: ijima@chem-eng.kyushu-u.ac.jp.
⁸ Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, Motooka 744, Nishi-ku, Fukuoka 819-0395, Japan; Division of Biotechnology, Center for Future Chemistry, Kyushu University, Japan. Electronic address: kamiya.noriho.367@m.kyushu-u.ac.jp.

PMID: 31276761
DOI: 10.1016/j.ijpharm.2019.118495

Abstract

We developed a new oil-based delivery system for transdermal protein delivery, a gel-in-oil (G/O) nanosuspension, where gelatin-based hydrogel was coated with hydrophobic surfactants. The high entrapment efficiency of a model protein, phycocyanin (PC), into nano-sized gelatin hydrogel particles was achieved. Spectroscopic evaluation of PC suggested that the G/O nanosuspension could retain the functional form of PC in isopropyl myristate. In vitro skin permeation studies showed that the G/O nanosuspension facilitated the delivery of PC through the stratum corneum of Yucatan micropig skin.

Keywords: Gelatin; Phycocyanin; Protein; Transdermal delivery.

MeSH terms

Administration, Cutaneous
Animals
Drug Carriers / administration & dosage*
Female
Gelatin / administration & dosage*
Hydrogels / administration & dosage*
Myristates / administration & dosage*
Nanoparticles / administration & dosage*
Oils / administration & dosage
Particle Size
Phycocyanin / administration & dosage*
Skin / metabolism
Skin Absorption
Swine
Swine, Miniature

Substances

Drug Carriers
Hydrogels
Myristates
Oils
isopropyl myristate
Phycocyanin
Gelatin