Ultrasensitive ciprofloxacin assay based on the use of a fluorescently labeled aptamer and a nanocomposite prepared from carbon nanotubes and MoSe2

Xiaobing Hu; Pengju Wei; Gaëlle Catanante; Zhanhong Li; Jean Louis Marty; Zhigang Zhu

doi:10.1007/s00604-019-3629-9

Ultrasensitive ciprofloxacin assay based on the use of a fluorescently labeled aptamer and a nanocomposite prepared from carbon nanotubes and MoSe₂

Mikrochim Acta. 2019 Jul 3;186(8):507. doi: 10.1007/s00604-019-3629-9.

Authors

Xiaobing Hu^{1

2}, Pengju Wei¹, Gaëlle Catanante³, Zhanhong Li¹, Jean Louis Marty³, Zhigang Zhu^{4

5}

Affiliations

¹ School of Environmental and Materials Engineering, College of Engineering, Shanghai Polytechnic University, Shanghai, 201209, China.
² School of Microelectronics, Fudan University, Shanghai, 200433, China.
³ BAE Laboratory, Université de Perpignan Via Domitia, 52 Avenue Paul Alduy, 66860, Perpignan, France.
⁴ School of Environmental and Materials Engineering, College of Engineering, Shanghai Polytechnic University, Shanghai, 201209, China. zgzhu@sspu.edu.cn.
⁵ Research Center of Resource Recycling Science and Engineering, Shanghai Ploytechnic University, Shanghai, 201209, China. zgzhu@sspu.edu.cn.

PMID: 31270699
DOI: 10.1007/s00604-019-3629-9

Abstract

A nanocomposite was prepared from carbon nanotubes and MoSe₂ (CNT-MoSe₂). This nanomaterial quenches the fluorescence of fluorescein-labeled aptamers. When ciprofloxacin (CIP) binds to the aptamer, an aptamer/G-quadruplex complex will be formed and the interaction between labeled aptamer and CNT-MoSe₂ nanostructures is weakened. This leads to significant fluorescence recovery. Under optimized experimental conditions, the limit of detection is 0.63 ng mL^-1 with a good linearity in the range from 0.63 to 80 ng mL^-1. The assay was applied to the determination of CIP in spiked milk, and the recoveries range between 94.3 and 97.0% (n = 3). Conceivably, the method is a generic approach that can be extended to the determination of other analyte for which adequate aptamers are available. Graphical abstract Schematic presentation of CNT-MoSe₂ quenching based aptamer assay for the detection of ciprofloxacin. The assay exhibits good selectivity, stability and reproducibility, and low limit of detection.

Keywords: Aptamer assay; CNT-MoSe2 nanostructure; Ciprofloxacin detection; Fluorescence quenching; Milk analysis.

Publication types

Research Support, Non-U.S. Gov't

Grants and funding

61471233/the National Natural Science Foundation of China/International