Methylomes of Two Extremely Halophilic Archaea Species, Haloarcula marismortui and Haloferax mediterranei

Shiladitya DasSarma; Alexey Fomenkov; Satyajit L DasSarma; Tamas Vincze; Priya DasSarma; Richard J Roberts

doi:10.1128/MRA.00577-19

Methylomes of Two Extremely Halophilic Archaea Species, Haloarcula marismortui and Haloferax mediterranei

Microbiol Resour Announc. 2019 Jul 3;8(27):e00577-19. doi: 10.1128/MRA.00577-19.

Authors

Shiladitya DasSarma¹, Alexey Fomenkov², Satyajit L DasSarma¹, Tamas Vincze², Priya DasSarma¹, Richard J Roberts³

Affiliations

¹ Institute of Marine and Environmental Technology, Department of Microbiology and Immunology, University of Maryland-Baltimore, Baltimore, Maryland, USA.
² New England Biolabs, Ipswich, Massachusetts, USA.
³ New England Biolabs, Ipswich, Massachusetts, USA roberts@neb.com.

Abstract

The genomes of two extremely halophilic Archaea species, Haloarcula marismortui and Haloferax mediterranei, were sequenced using single-molecule real-time sequencing. The ∼4-Mbp genomes are GC rich with multiple large plasmids and two 4-methyl-cytosine patterns. Methyl transferases were incorporated into the Restriction Enzymes Database (REBASE), and gene annotation was incorporated into the Haloarchaeal Genomes Database (HaloWeb).