Objective: To explore the relationship between polymorphisms of interleukin 17(IL-17) gene (rs4711998, rs763780) and the susceptibility of pneumoconiosis, and to provide a basis for prevention of high-risk groups of pneumoconiosis. Methods: A total of 219 pneumoconiosis patients and 242 workers without pneumoconiosis were enrolled in the study. All subjects were photographed with high undulating X-rays anterior chest radiographs, diagnosed according to diagnostic criteria for pneumoconiosis. We collected 3 ml of peripheral venous blood of the study subjects. Polymorphism in IL-17A rs4711998 and IL-17F rs763780 locus were evaluated by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results: The IL-17A rs4711998 locus has AA, AG and GG genotypes, there was no the significant difference between case and control groups (P>0.05). IL-17F rs763780 had AA, AG and GG genotypes, there was a significant difference between case and control groups (P<0.05). Allele A and allele G were statistically significant difference between the case group and the control group (P<0.05). Conclusion: No relationship was found between IL-17A gene polymorphisms at rs4711998 and silicosis. IL-17F rs763780 locus gene polymorphism is associated with susceptibility to pneumoconiosis. AG genotype and G allele may have a protective effect.
目的： 探讨白细胞介素17（IL-17）rs4711998、rs763780基因多态性与尘肺易感性的关系，为尘肺病高危人群的预防提供依据。 方法： 选择219例尘肺病患者和242例接尘者为研究对象，拍摄X射线高千伏后前位胸片，按尘肺病诊断标准进行诊断；采集研究对象外周静脉血3 ml，应用PCR-RFLP技术检测IL-17基因多态性。 结果： IL-17 A的rs4711998位点分为AA、AG和GG基因型，3种基因型在两组研究对象中的分布差异无统计学意义（P>0.05）。IL-17 F的rs763780位点分为AA、AG和GG基因型，3种基因型在两组中的分布差异有统计学意义（P<0.05），等位基因G、等位基因A在两组的差异均有统计学意义（P<0.05）。 结论： IL-17A rs4711998位点基因多态性可能与尘肺病的易感性不相关；IL-17F rs763780位点基因多态性可能与尘肺病的易感性相关，尘肺的保护基因型是AG，尘肺的保护等位基因是G等位基因。.
Keywords: Interleukin-17; Pneumoconiosis; Polymorphisms.