One immunoassay probe makes SERS and fluorescence two readout signals: Rapid imaging and determination of intracellular glutathione levels

Wenhuan Yao; Lu Chang; Weidong Yin; Tao Wang; Yuan Yang; Pei Yin; Manyi Yang; Yucheng Ma; Yanfang Qin; Hengchang Ma

doi:10.1016/j.saa.2019.117303

One immunoassay probe makes SERS and fluorescence two readout signals: Rapid imaging and determination of intracellular glutathione levels

Spectrochim Acta A Mol Biomol Spectrosc. 2019 Dec 5:223:117303. doi: 10.1016/j.saa.2019.117303. Epub 2019 Jun 22.

Authors

Wenhuan Yao¹, Lu Chang², Weidong Yin², Tao Wang², Yuan Yang², Pei Yin², Manyi Yang², Yucheng Ma², Yanfang Qin², Hengchang Ma³

Affiliations

¹ College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070, China. Electronic address: whyao@nwnu.edu.cn.
² College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070, China.
³ College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070, China. Electronic address: mahczju@hotmail.com.

PMID: 31255857
DOI: 10.1016/j.saa.2019.117303

Abstract

In this paper, one probe (TPPA-VCh) with fluorescence and Surface-enhanced Raman Scattering (SERS) two readout signals, which has high sensitivity and specificity to glutathione in both vitro and cell image applications, is designed and synthesized. Furthermore, the quenched emissions and intensified SERS signals is obtained by loading TPPA-VCh on the surfaces of gold nanoparticles.

Keywords: Aggregation-induced emission; Immunoassay probe; Rapid cellular imaging; Surface-enhanced Raman Scattering.

MeSH terms

Glutathione / analysis*
Gold / chemistry
Hep G2 Cells
Humans
Immunoassay / methods*
Intracellular Space / metabolism*
Metal Nanoparticles / chemistry
Metal Nanoparticles / ultrastructure
Molecular Imaging*
Molecular Probes / chemistry*
Particle Size
Spectrometry, Fluorescence
Spectrum Analysis, Raman / methods*

Substances

Molecular Probes
Gold
Glutathione