A sensitive and radiolabeling-free method for pseudouridine detection

Wei Li; Fang Wang; Yi Chen; Xiaocheng Weng; Xiang Zhou

doi:10.1016/j.ab.2019.113350

A sensitive and radiolabeling-free method for pseudouridine detection

Anal Biochem. 2019 Sep 15:581:113350. doi: 10.1016/j.ab.2019.113350. Epub 2019 Jun 27.

Authors

Wei Li¹, Fang Wang², Yi Chen¹, Xiaocheng Weng³, Xiang Zhou¹

Affiliations

¹ College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, The Institute for Advanced Studies, Hubei Province Key Laboratory of Allergy and Immunology, Wuhan University, Wuhan, Hubei, 430072, PR China.
² Wuhan University School of Pharmaceutical Sciences, Wuhan University, Wuhan, 430071, China.
³ College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, The Institute for Advanced Studies, Hubei Province Key Laboratory of Allergy and Immunology, Wuhan University, Wuhan, Hubei, 430072, PR China. Electronic address: xcweng@whu.edu.cn.

PMID: 31255565
DOI: 10.1016/j.ab.2019.113350

Abstract

Existing methodologies for detecting Pseudouridine (Ψ) mostly use CMCT labeling or radiolabeling. Described herein is a sensitive and quantitative method for Ψ detection that does not need this labelling. This approach combines the selectivity of a 10-23 DNAzyme, which can distinguish Ψ from uridine (U), with rolling circle amplification (RCA) to increase the sensitivity of the assay.

Keywords: DNAzyme; Pseudouridine (Ψ); Rolling circle amplification (RCA).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA, Catalytic / chemistry*
Pseudouridine* / analysis
Pseudouridine* / metabolism
RNA, Fungal* / analysis
RNA, Fungal* / metabolism
Saccharomyces cerevisiae / metabolism*

Substances

DNA, Catalytic
RNA, Fungal
Pseudouridine