Effects of cerium oxide on rice seedlings as affected by co-exposure of cadmium and salt

Abstract

Effects of CeO₂ NPs (200 mg.L^-1) on rice (Oryza sativa L.) alone or co-exposure with cadmium (Cd) and salt (sodium chloride, NaCl) were investigated in hydroponic systems for two weeks. Physiological results show that rice biomass was significantly inhibited when NaCl or CdCl₂ added alone or in co-exposure treatment. CeO₂ NPs significantly relieve the chlorophyll damage under CdCl₂ environmental stress. The presence of CeO₂ NPs alleviated both stressors induced damages to rice as indicated by the reduced proline level. Additionally, CeO₂ NPs triggered the antioxidant defense systems to counteract the oxidative stress caused by NaCl and CdCl₂. The level of 8-OHdG, one of the most important indicators for genotoxicity, in rice suggest that the presence of CeO₂ NPs reduced the DNA damage in NaCl treated rice. Elemental analysis indicated that co-exposure to NaCl and CdCl₂ slightly decreased the Cd content as compared to the one in the CdCl₂ alone treatment, and this co-exposure also significantly reduced the Na content when comparing with the NaCl alone treatment. Taken together, our findings suggest that CeO₂ NPs could alleviate the CdCl₂ and NaCl stresses, but could not completely change the phenotype of both contaminants treated rice.

Keywords: 8-OHdG; Antioxidant enzymes; Cadmium; Co-contamination; Oryza sativa L.; Salt.