Super-resolution Microscopy of Vaccinia Virus Particles

Robert Gray; David Albrecht

doi:10.1007/978-1-4939-9593-6_16

Super-resolution Microscopy of Vaccinia Virus Particles

Methods Mol Biol. 2019:2023:255-268. doi: 10.1007/978-1-4939-9593-6_16.

Authors

Robert Gray¹, David Albrecht²

Affiliations

¹ MRC Laboratory for Molecular Cell Biology, University College London, London, UK. robert.gray.14@ucl.ac.uk.
² MRC Laboratory for Molecular Cell Biology, University College London, London, UK. d.albrecht@ucl.ac.uk.

PMID: 31240683
DOI: 10.1007/978-1-4939-9593-6_16

Abstract

Super-resolution microscopy enables the study of vaccinia architecture at subviral resolution with molecular specificity. Here, we outline how to use structured illumination microscopy (SIM) and stochastic optical reconstruction microscopy (STORM) to detect fluorescently tagged or immunolabeled viral proteins on purified virions. Tens to hundreds of individual virions can be imaged in a single field of view providing data for single-particle averaging or quantitative analysis of viral protein spatial organization.

Keywords: Immunofluorescence; SIM; STORM; Super-resolution microscopy; Vaccinia virus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Fluorescent Antibody Technique
Microscopy, Fluorescence / methods*
Vaccinia virus / genetics*
Virion