Vaccinia Virus Genome Editing Using CRISPR

Carmela Di Gioia; Ming Yuan; Yaohe Wang

doi:10.1007/978-1-4939-9593-6_6

Vaccinia Virus Genome Editing Using CRISPR

Methods Mol Biol. 2019:2023:109-117. doi: 10.1007/978-1-4939-9593-6_6.

Authors

Carmela Di Gioia¹, Ming Yuan¹, Yaohe Wang²

Affiliations

¹ Barts Cancer Institute, Queen Mary University, London, UK.
² Barts Cancer Institute, Queen Mary University, London, UK. yaohe.wang@qmul.ac.uk.

PMID: 31240673
DOI: 10.1007/978-1-4939-9593-6_6

Abstract

CRISPR/Cas9, an RNA-guided targeted genome editing system, can make precise, targeted modifications to the genome in living cells. Here we describe how this method can be used to efficiently edit the vaccinia virus genome enabling the insertion of transgene(s) specifically into a targeted site.

Keywords: CRISPR; Cas9; Editing; Genome; Homologous recombination; Vaccinia virus.

MeSH terms

CRISPR-Associated Protein 9 / genetics
Clustered Regularly Interspaced Short Palindromic Repeats / genetics
Gene Editing / methods
Genome, Viral / genetics*
Vaccinia virus / genetics*

Substances

CRISPR-Associated Protein 9