Generation of an integration-free human induced pluripotent stem cell line MUSIi010-A from occipital scalp fibroblasts of a male patient with androgenetic alopecia

Nutnicha Tantarungsee; Zaukir Bostan Ali; Rungtip Soi-Ampornkul; Chuda Rujitharanawong; Chanika Subchookul; Alisa Ritthaphai; Bootsakorn Boonkaew; Methichit Wattanapanitch; Kanchalit Thanomkitti; Daranporn Triwongwaranat; Chinnavuth Vatanashevanopakorn; Rattapon Thuangtong

doi:10.1016/j.scr.2019.101486

Generation of an integration-free human induced pluripotent stem cell line MUSIi010-A from occipital scalp fibroblasts of a male patient with androgenetic alopecia

Stem Cell Res. 2019 Jul:38:101486. doi: 10.1016/j.scr.2019.101486. Epub 2019 Jun 14.

Affiliations

¹ Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand; Siriraj Center for Regenerative Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
² Department of Dermatology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
³ Siriraj Center for Regenerative Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
⁴ Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand; Siriraj Center for Regenerative Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand. Electronic address: chinnavuth.vat@mahidol.ac.th.
⁵ Department of Dermatology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand. Electronic address: rattaponthuangtong@yahoo.com.

PMID: 31228683
DOI: 10.1016/j.scr.2019.101486

Abstract

An induced pluripotent stem cell (iPSC) line, MUSIi010-A, was established by Sendai virus (SeV) transduction of scalp fibroblasts from a 59-year-old male with androgenetic alopecia (AGA). Pluripotency of the iPSC line was verified by immunofluorescence staining of pluripotent markers and by in vitro trilineage differentiation. The MUSIi010-A line was shown to retain normal karyotype and free of SeV vectors at passage 17. This iPSC line can be used for studying pathological mechanisms of AGA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alopecia* / metabolism
Alopecia* / pathology
Cell Line
Cellular Reprogramming Techniques*
Fibroblasts* / metabolism
Fibroblasts* / pathology
Humans
Induced Pluripotent Stem Cells* / metabolism
Induced Pluripotent Stem Cells* / pathology
Male
Middle Aged
Scalp* / metabolism
Scalp* / pathology