In vivo effect of bevacizumab-loaded albumin nanoparticles in the treatment of corneal neovascularization

Exp Eye Res. 2019 Aug:185:107697. doi: 10.1016/j.exer.2019.107697. Epub 2019 Jun 19.

Abstract

Corneal neovascularization (CNV) is associated with different ocular pathologies, including infectious keratitis, trachoma or corneal trauma. Pharmacological treatments based on the topical application of anti-VEGF therapies have been shown to be effective in the treatment and prevention of CNV. The aim of this work was to evaluate the effect of bevacizumab-loaded albumin nanoparticles in a rat model of CNV. Bevacizumab-loaded nanoparticles, either "naked" (B-NP) or coated with PEG 35,000 (B-NP-PEG), were administered once a day in the eyes of animals (10 μL, 4 mg/mL every 24 h) during 7 days. Bevacizumab and dexamethasone were employed as controls and administered at the same dose every 12 h. At the end of the study, the area of the eye affected by neovascularization was about 2-times lower for animals treated with B-NP than with free bevacizumab. In the study, dexamethasone did not demonstrate an inhibitory effect on CNV at the employed dose. All of these results were confirmed by histopathological analysis, which clearly showed that eyes treated with nanoparticles displayed lower levels of fibrosis, inflammation and edema. In summary, the encapsulation of bevacizumab in human serum albumin nanoparticles improved its efficacy in an animal model of CNV.

Keywords: Bevacizumab; Corneal neovascularization; Human serum albumin; Nanoparticles; Ocular delivery; Pegylated.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Angiogenesis Inhibitors / therapeutic use*
  • Animals
  • Bevacizumab / therapeutic use*
  • Coated Materials, Biocompatible
  • Corneal Neovascularization / drug therapy*
  • Corneal Neovascularization / pathology
  • Disease Models, Animal*
  • Drug Carriers / chemistry*
  • Male
  • Nanoparticles / chemistry*
  • Polyethylene Glycols
  • Rats
  • Rats, Wistar
  • Serum Albumin, Human / chemistry*
  • Vascular Endothelial Growth Factor A / antagonists & inhibitors

Substances

  • Angiogenesis Inhibitors
  • Coated Materials, Biocompatible
  • Drug Carriers
  • Vascular Endothelial Growth Factor A
  • vascular endothelial growth factor A, rat
  • Bevacizumab
  • Polyethylene Glycols
  • Serum Albumin, Human