Evaluation of a Screening Method for the Detection of Colistin-Resistant Enterobacteriaceae in Stool

Sarah E Turbett; Lisa Desrosiers; Catherine Andrews-Dunleavey; Margaret Becker; Allison Taylor Walker; Douglas Esposito; Kate Russell Woodworth; John A Branda; Eric Rosenberg; Edward T Ryan; Regina LaRocque

doi:10.1093/ofid/ofz211

Evaluation of a Screening Method for the Detection of Colistin-Resistant Enterobacteriaceae in Stool

Open Forum Infect Dis. 2019 May 6;6(6):ofz211. doi: 10.1093/ofid/ofz211. eCollection 2019 Jun.

Authors

Sarah E Turbett^{1

2

3}, Lisa Desrosiers³, Catherine Andrews-Dunleavey³, Margaret Becker³, Allison Taylor Walker⁴, Douglas Esposito⁴, Kate Russell Woodworth⁵, John A Branda^{2

3}, Eric Rosenberg^{1

2

3}, Edward T Ryan^{1

2

6}, Regina LaRocque^{1

2}

Affiliations

¹ Department of Medicine, Massachusetts General Hospital, Boston, Massachusetts.
² Department of Medicine, Harvard Medical School, Boston, Massachusetts.
³ Department of Pathology, Massachusetts General Hospital, Boston, Massachusetts.
⁴ Travelers' Health Branch, Division of Global Migration and Quarantine, Centers for Disease Control and Prevention, Atlanta, Georgia.
⁵ Prevention and Response Branch, Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia.
⁶ Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, Massachusetts.

Abstract

Emergence of mobile colistin resistance (mcr)-containing Enterobacteriaceae is a public health threat, prompting enhanced surveillance through the Centers for Disease Control and Prevention. We evaluated a selective culture medium for the isolation of Enterobacteriaceae with non-wild-type colistin minimum inhibitory concentrations, including those with mcr-1 genes, in spiked stool samples.

Keywords: colistin resistance; mcr Enterobacteriaceae; screening.

Abstract

Grants and funding