Co3O4 nanoparticles supported mesoporous carbon framework interface for glucose biosensing

Abstract

The present work reports the preparation of advanced functional nanostructures based on cobalt oxide supported mesoporous carbon framework (Co₃O₄@MCF) for electrochemical biosensing. Co₃O₄@MCF was synthesized by simple hythrothermal & pyrolysis method and further characterized by various microscopic and spectroscopic techniques. The transmission electron microscopic (TEM) images show the lattice fringes of crystalline Co₃O₄ with interlayer spacing of 0.24 nm. The characteristic 311 plane in X-ray diffraction (XRD) studies further confirmed the presence of crystalline Co₃O₄ on carbon frameworks. Reflection of prominent A1g peak along with D and G band in raman spectra confirmed the successful fabrication of Co₃O₄@MCF nanocomposite. Prepared Co₃O₄@MCF manifested great porosity, good biocompatibility and large surface area which allowed effective immobilization of glucose oxidase (GOx) onto its surface using chitosan (Chi) as a binder. Thus, a nanocomposite (Co₃O₄@MCF-Chi-GOx) modified glassy carbon electrode (GCE) was fabricated for highly selective detection of glucose using amperometry and cyclic voltammetry. The Co₃O₄@MCF-Chi-GOx/GCE electrode exhibited excellent biosensing performance for glucose monitoring with detection limit of (LOD) of 107.70 μM and reproducibility of 4.7% RSD. Moreover, the biosensor holds great promise for its effective implications in point-of-care diagnostics of small biomolecules.

Keywords: Amperometry; Biosensors; Cobalt oxide supported mesoporous carbon framework (Co(3)O(4)@MCF); Glucose; Glucose oxidase.