Activation of fulvic acid-like in paper mill effluents using H2O2/TiO2 catalytic oxidation: Characterization and salt stress bioassays

Abstract

Increasing environmental concerns about organic waste in paper mill effluents demand alternative wastewater management technology. We reported novel activation of fulvic acid-like in paper mill effluents using hydrogen peroxide (H₂O₂) as oxidizer and titanium oxide (TiO₂) as catalyst. Spectroscopic characteristics of fulvic acid-like in paper mill effluents before and after activation (PFA and PFA-Os, respectively) were compared with a benchmark fulvic acid extracted from leonardite (LFA). Results indicated that PFA-Os exhibited less lignin structures, more functional groups and lower molecular weight than PFA, sharing much similarity with LFA. Among PFA-Os with varying degrees of oxidation, PFA-O-3 activated with 1:2 vol ratio of paper mill effluent and 30% H₂O₂ for 20 min digestion at 90 °C stands out to be the optimal for further examination of its biological activity. Bioassays with rice seed/seedling indicated that applications of LFA at 2-5 mg-C/L and PFA-O-3 at 60-100 mg-C/L significantly increased rice seed germination rate and seedling growth under salt stress imposed with 100 mM NaCl. The mechanism was mainly through reduced oxidative damage via activation of antioxidative enzymes and lipid peroxidation. This study provides the needed technical basis of safer and cleaner technologies for innovative management of paper mill effluents.

Keywords: Antioxidative enzymes; Fulvic acid-like; Paper mill effluents; Rice seedling; Salt stress.