As, Cd, and Pb relative bioavailability in contaminated soils: Coupling mouse bioassay with UBM assay

Xia Zhu; Meng-Ya Li; Xiao-Qiang Chen; Jue-Yang Wang; Lian-Zhen Li; Chen Tu; Yong-Ming Luo; Hong-Bo Li; Lena Q Ma

doi:10.1016/j.envint.2019.05.069

As, Cd, and Pb relative bioavailability in contaminated soils: Coupling mouse bioassay with UBM assay

Environ Int. 2019 Sep:130:104875. doi: 10.1016/j.envint.2019.05.069. Epub 2019 Jun 11.

Authors

Xia Zhu¹, Meng-Ya Li², Xiao-Qiang Chen², Jue-Yang Wang², Lian-Zhen Li³, Chen Tu³, Yong-Ming Luo⁴, Hong-Bo Li⁵, Lena Q Ma⁶

Affiliations

¹ CAS Key Laboratory of Coastal Environmental Process and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China; University of Chinese Academy of Sciences, Beijing 100049, China.
² State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, China.
³ CAS Key Laboratory of Coastal Environmental Process and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China.
⁴ CAS Key Laboratory of Coastal Environmental Process and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China; University of Chinese Academy of Sciences, Beijing 100049, China; Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China. Electronic address: ymluo@yic.ac.cn.
⁵ State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, China. Electronic address: hongboli@nju.edu.cn.
⁶ State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, China; Soil and Water Science Department, University of Florida, Gainesville, FL 32611, USA.

PMID: 31200159
DOI: 10.1016/j.envint.2019.05.069

Abstract

The robustness of in vitro bioaccessibility assays to predict oral relative bioavailability (RBA) of multiple metals in contaminated soils requires validation using additional soil samples. In this study, 11 contaminated soils from mining/smelting areas were analyzed for As-, Cd-, and Pb-RBA using a mouse bioassay and metal bioaccessibility via the UBM gastric phase assay. Metal-RBA varied considerably among soils, with As-RBA (2.5-23%, mean 12%) being generally lower than Cd-and Pb-RBA (3.4-88 and 3.3-59%, mean 42 and 28%), due to higher proportions of As in the residual fractions. Metal-RBA generally decreased with increasing metal concentrations probably due to reduced labile metal fractions. In addition, strong negative correlations were observed between total Fe with As-, Cd-, and Pb-RBA (R² = 0.46-0.77), suggesting the role of Fe in controlling metal-RBA in soils. Like RBA, metal bioaccessibility by the UBM assay also varied among samples. However, strong in vivo-in vitro correlations (IVIVCs) were observed between metal-RBA and bioaccessibility (R² = 0.52-0.81). Further, there were little differences when As-, Cd-, and Pb-IVIVCs established using soils from this study and soils pooled from literature were compared, suggesting the robustness of the UBM assay to predict metal-RBA in contaminated soils.

Keywords: Bioaccessibility; Bioavailability; Heavy metals; In vitro assay; Mouse model.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Arsenic / analysis
Arsenic / pharmacokinetics*
Biological Assay / methods*
Biological Availability
Cadmium / analysis
Cadmium / pharmacokinetics*
Female
Lead / analysis
Lead / pharmacokinetics*
Mice, Inbred BALB C
Mining
Soil Pollutants / analysis
Soil Pollutants / pharmacokinetics*

Substances

Soil Pollutants
Cadmium
Lead
Arsenic