The PI3KCA and AKT Inhibitory Activities of Litsea Cubeba Lour. Fruits and Heartwoods Towards Hela Cells

Aminah Dalimunthe; Poppy Anjelisa Zaitun Hasibuan; Denny Satria

doi:10.3889/oamjms.2019.317

The PI3KCA and AKT Inhibitory Activities of Litsea Cubeba Lour. Fruits and Heartwoods Towards Hela Cells

Open Access Maced J Med Sci. 2019 May 14;7(9):1422-1424. doi: 10.3889/oamjms.2019.317. eCollection 2019 May 15.

Authors

Aminah Dalimunthe¹, Poppy Anjelisa Zaitun Hasibuan¹, Denny Satria²

Affiliations

¹ Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155, Indonesia.
² Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155, Indonesia.

Abstract

Aim: To investigated the activities of chloroform fractions at pH 7 of Litsea cubeba Lour. Fruits and heartwoods (CF-7F and CF-7H) in decrease expression of PI3KCA, Akt-1 and Akt-2 genes towards cervical cancer cell culture (HeLa) experiments in vitro.

Material and methods: CF-7F and CF-7H (12.5 and 25 µg/mL) were tested for its potential inhibition on gene expression of PI3KCA, Akt-1 and Akt-2 genes by Reverse Transcription-Polymerase Chain Reaction (RT-PCR) method.

Result: CF-7F and CF-7H were showed the activity to reduce the expression of PI3KCA, Akt-1 and Akt-2 genes.

Conclusion: Our results suggest that CF-7F and CF-7H significantly inhibit the expression of PI3KCA, Akt-1 and Akt-2 genes.

Keywords: Akt; Fruits; Heartwoods; Litsea cubeba Lour; PI3KCA.