Seven isolates of Trichoderma spp. (T1 to T7) from Egypt were evaluated in vitro by bioassay for their potential to antagonize Fusarium oxysporum f.sp. lycopersici (FOL, the causal pathogen of tomato wilt disease). The highest percentage of inhibition against the tested pathogenic isolate were obtained with Trichoderma isolate (T7) followed by Trichoderma isolate (T3). In greenhouse experiments, the application of the highly antagonistic isolates of Trichoderma spp. (T3 and T7) led to a significant decrease of disease severity compared to the untreated control treatment. The lowest severity was achieved with the T3 isolate (24.8%) followed by isolate T7 (34.6%) compared with the other tested isolates. To understand the ability of Trichoderma isolates to protect against wilt disease, its induced systemic resistance in tomato plants has been studied. The expression of a defense-related gene (β-1,3-glucanase gene) was assessed by real-time RT-PCR in tomato plants to test the accumulation kinetics of transcripts encoding PR proteins in the roots of tomato in control (only with the pathogen), T3&FOL, and T7&FOL treatments. The highest degree of gene expression was found in tomato plants which were treated with T3&FOL compared with control (pathogen only). Two species of antagonistic Trichoderma (T3& T7) were characterized based on molecular tools using internal transcribed spacers (ITS1 and ITS4). The results of genetic characterization identified two different species of Trichoderma (T. atroviride and T. longibrachiatum).
Keywords: Induced resistance; Internal transcribed spacer (ITS); Real-time RT-PCR; Tomato wilt disease.