Self-Assembly of Wireframe DNA Nanostructures from Junction Motifs

Kai Huang; Donglei Yang; Zhenyu Tan; Silian Chen; Ye Xiang; Yongli Mi; Chengde Mao; Bryan Wei

doi:10.1002/anie.201906408

Self-Assembly of Wireframe DNA Nanostructures from Junction Motifs

Angew Chem Int Ed Engl. 2019 Aug 26;58(35):12123-12127. doi: 10.1002/anie.201906408. Epub 2019 Jul 24.

Authors

Kai Huang¹, Donglei Yang^{2

3}, Zhenyu Tan^{1

4}, Silian Chen^{5

6}, Ye Xiang⁵, Yongli Mi^{2

7}, Chengde Mao⁸, Bryan Wei¹

Affiliations

¹ School of Life Sciences, Tsinghua University-Peking University Center for Life Sciences, Center for Synthetic and Systems Biology, Tsinghua University, Beijing, 100084, China.
² School of Chemical Science and Engineering, Tongji University, Shanghai, 200092, China.
³ Institute of Molecular Medicine (IMM), Renji Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai, 200127, China.
⁴ Present address: Biophysics Program, University of Michigan, Ann Arbor, MI, 48109, USA.
⁵ Center for Infectious Disease Research, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Beijing Advanced Innvation Center for Structural Biology, Department of Basic Medical Sciences, School of Medicine, Tsinghua University, Beijing, 100084, China.
⁶ School of Life Sciences, Peking University, Beijing, 100084, China.
⁷ Department of Chemical and Biological Engineering, Hong Kong University of Science and Technology, Kowloon, Hong Kong SAR, China.
⁸ Department of Chemistry, Purdue University, West Lafayette, IN, 47907, USA.

PMID: 31190457
DOI: 10.1002/anie.201906408

Abstract

Wireframe frameworks have been investigated for the construction of complex nanostructures from a scaffolded DNA origami approach; however, a similar framework is yet to be fully explored in a scaffold-free "LEGO" approach. Herein, we describe a general design scheme to construct wireframe DNA nanostructures entirely from short synthetic strands. A typical edge of the resulting structures in this study is composed of two parallel duplexes with crossovers on both ends, and three, four, or five edges radiate out from a certain vertex. By using such a self-assembly scheme, we produced planar lattices and polyhedral objects.

Keywords: DNA nanostructures; junction motifs; self-assembly; wireframe structures.

Publication types

Research Support, Non-U.S. Gov't

Abstract

Publication types

Grants and funding