Rapid Increase in Carbapenemase-Producing Enterobacteriaceae in Retail Meat Driven by the Spread of the blaNDM-5-Carrying IncX3 Plasmid in China from 2016 to 2018

Qianhui Zhang; Luchao Lv; Xiuyu Huang; Ying Huang; Zilin Zhuang; Jiaxun Lu; Enyu Liu; Miao Wan; Haoliang Xun; Zhiwei Zhang; Jin Huang; Qianhua Song; Chao Zhuo; Jian-Hua Liu

doi:10.1128/AAC.00573-19

Rapid Increase in Carbapenemase-Producing Enterobacteriaceae in Retail Meat Driven by the Spread of the bla_NDM-5-Carrying IncX3 Plasmid in China from 2016 to 2018

Antimicrob Agents Chemother. 2019 Jul 25;63(8):e00573-19. doi: 10.1128/AAC.00573-19. Print 2019 Aug.

Authors

Qianhui Zhang^#^{1

2}, Luchao Lv^#^{1

2}, Xiuyu Huang^{1

2}, Ying Huang^{1

2}, Zilin Zhuang^{1

2}, Jiaxun Lu^{1

2}, Enyu Liu^{1

2}, Miao Wan^{1

2}, Haoliang Xun^{1

2}, Zhiwei Zhang^{1

2}, Jin Huang^{1

2}, Qianhua Song^{1

2}, Chao Zhuo³, Jian-Hua Liu^{4

2}

Affiliations

¹ College of Veterinary Medicine, National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, Key Laboratory of Zoonosis of Ministry of Agricultural and Rural Affairs, South China Agricultural University, Guangzhou, China.
² Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou, China.
³ State Key Laboratory of Respiratory Disease, First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
⁴ College of Veterinary Medicine, National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, Key Laboratory of Zoonosis of Ministry of Agricultural and Rural Affairs, South China Agricultural University, Guangzhou, China jhliu@scau.edu.cn.

^# Contributed equally.

Abstract

The presence and molecular characteristics of carbapenemase-producing Enterobacteriaceae (CPE) among meat products in China were investigated. A total of 110 carbapenem-resistant Enterobacteriaceae (CRE) isolates, including 94 Escherichia coli and 10 Klebsiella pneumoniae isolates, were identified from 105 of 794 (13.2%) samples. The positive rates markedly increased from 2016 (9.4%) to 2018 (22.2%). Only bla_NDM genes were detected; 79.1% of bla_NDM genes were carried by IncX3 plasmids. Routine monitoring of carbapenemase-producing Enterobacteriaceae in the animal food supply is highly recommended.

Keywords: China; Escherichia coli; blaNDM; food; plasmid.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Anti-Bacterial Agents / therapeutic use
Bacterial Proteins / genetics
Carbapenem-Resistant Enterobacteriaceae / genetics
Carbapenems / therapeutic use*
China
Escherichia coli / drug effects
Escherichia coli / genetics*
Escherichia coli Infections / drug therapy
Escherichia coli Infections / microbiology
Humans
Klebsiella Infections / drug therapy
Klebsiella Infections / metabolism
Klebsiella pneumoniae / drug effects
Klebsiella pneumoniae / genetics*
Meat / microbiology*
Microbial Sensitivity Tests / methods
Plasmids / genetics*
beta-Lactamases / genetics*

Substances

Anti-Bacterial Agents
Bacterial Proteins
Carbapenems
beta lactamase NDM-5, E coli
beta-Lactamases