Abstract
Transcription cofactor Yes-associated protein (YAP) plays an important role in cancer progression. Here, we found that Aurora A kinase expression was positively correlated with YAP in lung cancer. Aurora A depletion suppresses lung cancer cell colony formation, which could be reversed by YAP ectopic overexpression. In addition, activation of Aurora A increases YAP protein abundance through maintaining its protein stability. Consistently, the transcriptional activity of YAP is increased upon Aurora A activation. We further showed that shAURKA suppressed YAP expression in the absence of Lats1/2, indicating that Aurora A regulates YAP independently of Hippo pathway. Instead, Aurora A induced blockage of autophagy to up-regulate YAP expression. Collectively, our findings provide insights into regulatory mechanisms of YAP expression in lung cancer development.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Signal Transducing / genetics
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Adaptor Proteins, Signal Transducing / metabolism*
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Aurora Kinase A / genetics
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Aurora Kinase A / metabolism*
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Autophagy* / genetics
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Carcinoma, Non-Small-Cell Lung / enzymology
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Carcinoma, Non-Small-Cell Lung / genetics
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Carcinoma, Non-Small-Cell Lung / metabolism*
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Cell Line, Tumor
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Cell Movement / genetics
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Cell Proliferation / genetics
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Hippo Signaling Pathway
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Humans
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Lung Neoplasms / enzymology
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Lung Neoplasms / genetics
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Lung Neoplasms / metabolism*
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Proteasome Endopeptidase Complex / metabolism
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Protein Serine-Threonine Kinases / metabolism
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Protein Stability
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Signal Transduction / genetics
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Transcription Factors / genetics
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Transcription Factors / metabolism*
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YAP-Signaling Proteins
Substances
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Adaptor Proteins, Signal Transducing
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Transcription Factors
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YAP-Signaling Proteins
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YAP1 protein, human
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LATS1 protein, human
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AURKA protein, human
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Aurora Kinase A
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Protein Serine-Threonine Kinases
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Proteasome Endopeptidase Complex