Assessing key microbial communities determining nitrogen transformation in composting of cow manure using illumina high-throughput sequencing

Abstract

Insight to nitrogen transformation and cycling during composting is vital in developing management strategies that improve nitrogen content and quality of the end product. In this study, a positive ventilation device was constructed and used to elucidate nitrogen transformation and microbial community structures during the composting of cow manure and rice straw. Bacterial community successions were analyzed during the composting process by examining the change in their structural dynamics using high-throughput sequencing technique. The results revealed that dominant phyla, included Acidobacter, Proteobacteria, Firmicutes, Bacteroidetes, Chloroflexi, and Actinobacteria. Furthermore, a positive strong correlation was observed between the key bacterial communities and nitrogen transformation. Analyses of functional genera, Spearman correlation and Path showed that Thermomonospora_curvata_DSM_43183 followed by Luteimonas and Simiduia, Brevundimonas and Tamlana, Pseudomonas followed by Brevundimonas and Flavobacterium were the key bacterial communities affecting NH₄⁺-N, NO₃^--N, and NO₂^--N transformation, respectively. Thauera followed by Pseudomonas_putida_NBRC_14164 played a dominant role in N₂O transformation.

Keywords: Composting; Illumina high-throughput sequencing; Microbial community; Nitrogen transformation; Path analysis.