A novel long non-coding RNA TONSL-AS1 regulates progression of gastric cancer via activating TONSL

Exp Cell Res. 2019 Sep 1;382(1):111453. doi: 10.1016/j.yexcr.2019.05.034. Epub 2019 May 31.

Abstract

Long non-coding RNAs (lncRNAs) are reported to play a significant role in various malignant tumors, yet their potential functions in gastric cancer are not clear. In this study, we found a novel lncRNA, named TONSL-AS1, was downregulated in gastric cancer tissues and cell lines compared with the normal. TONSL-AS1 inhibited cell migration, invasion and proliferation in SGC-7901, MGC-803 cells. Furthermore, TONSL-AS1 could suppress cell tumorigenesis in vivo. Mechanistically, TONSL-AS1's genomic neighboring gene TONSL, which was reported as a tumor suppress gene, was upregulated by TONSL. Additionally, the TONSL-AS1 was positively associated with TONSL in cancer tissues. Our study revealed that the tumor-inhibiting effect of TONSL-AS1 in gastric cancer cells was associated with TONSL. In general, our results indicated that TONSL-AS1 works as a tumor suppressor lncRNA, which may be a new therapeutic target for gastric cancer.

Keywords: Gastric cancer; Long non-coding RNA; TONSL; TONSL-AS1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Animals
  • Carcinogenesis
  • Cell Adhesion
  • Cell Division
  • Cell Line, Tumor
  • Cell Movement
  • Disease Progression
  • Down-Regulation
  • Female
  • Gene Expression Regulation, Neoplastic / genetics*
  • Genes, Reporter
  • Genes, Tumor Suppressor*
  • Humans
  • Male
  • Mice, Inbred BALB C
  • Mice, Nude
  • Middle Aged
  • NF-kappa B / genetics
  • NF-kappa B / physiology*
  • Neoplasm Proteins / genetics
  • Neoplasm Proteins / physiology*
  • RNA Interference
  • RNA, Neoplasm / genetics
  • RNA, Neoplasm / physiology*
  • RNA, Small Interfering / genetics
  • RNA, Small Interfering / pharmacology
  • Specific Pathogen-Free Organisms
  • Stomach Neoplasms / genetics*

Substances

  • NF-kappa B
  • Neoplasm Proteins
  • RNA, Neoplasm
  • RNA, Small Interfering
  • TONSL protein, human