A novel microbiological medium for the growth of periodontitis associated pathogens

Niall A Hickey; Kathryn A Whitehead; Liliana Shalamanova; Jonathan A Butler; Rebecca L Taylor

doi:10.1016/j.mimet.2019.105647

A novel microbiological medium for the growth of periodontitis associated pathogens

J Microbiol Methods. 2019 Aug:163:105647. doi: 10.1016/j.mimet.2019.105647. Epub 2019 May 30.

Authors

Niall A Hickey¹, Kathryn A Whitehead¹, Liliana Shalamanova¹, Jonathan A Butler¹, Rebecca L Taylor²

Affiliations

¹ Department of Life Sciences, Faculty of Science and Engineering, Manchester Metropolitan University, Chester Street, Manchester M1 5GD, UK.
² Department of Life Sciences, Faculty of Science and Engineering, Manchester Metropolitan University, Chester Street, Manchester M1 5GD, UK. Electronic address: r.taylor@mmu.ac.uk.

PMID: 31153964
DOI: 10.1016/j.mimet.2019.105647

Abstract

A novel microbiological medium designed to be more representative of gingival crevicular fluid. Chosen representative periodontal microorganisms showed good growth with minimal effect on human cell viability. This will enable more comparisons between different periodontitis associated organisms and their potential role in host health and systemic disease.

Keywords: Cell culture; Chronic periodontitis; Growth medium; Oral microbiology.

MeSH terms

Bacteria / growth & development*
Cell Culture Techniques / methods
Chronic Periodontitis / microbiology*
Culture Media, Conditioned / chemistry*
Culture Media, Conditioned / pharmacology
Fibroblasts / drug effects
Gingival Crevicular Fluid / microbiology*
Humans
Primary Cell Culture

Substances

Culture Media, Conditioned