It is very important to examine carefully the potential adverse effects of engineered nanoparticles (NPs) on human health and environments. In the present study, we have investigated the impact of interfacial serum proteins on the cell membrane disruption induced by silica NPs of primary diameter of 55-68 nm in four types of cells (erythrocytes, Jurkat, B16F10, and J774.1). The silica-induced membranolysis was repressed by addition of 1-2% serum into culture media, where the adhesion amount of the FBS-coated silica NPs onto a cell surface seemed comparable with that of the bare silica NPs. The nonspecific attraction between the bare silica and J774.1 cell membrane surfaces was masked by pretreatment of the silica surface with serum albumin, whereas the serum proteins-coated silica surface exhibited the attractive interactions with the cell membrane due to specific binding between some of adsorbed proteins thereon and the membrane receptors. The difference in silica-cell interaction between the nonspecific and specific attractions would explain the reason why interfacial serum proteins reduced the membranolysis without prevention of silica NPs adhering to cell surfaces.
Keywords: Hemolysis; Mammalian cells; Membrane disruption; Protein corona; Silica nanoparticles.
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