Baseline data on the insect successional patterns on carcasses can be a valuable estimation tool in the investigations of suspicious deaths, particularly when the post-mortem interval is longer than months or years. However, although carrion insect succession is a recurrent topic in forensic science research, the duration of the published studies is typically shorter than 1 year, with only one published study from central Europe documenting successional patterns beyond the first year of decomposition. We provide here the first data on the long-term insect successional patterns in southern Europe, using pig carcasses exposed in the four seasons of the year and documenting the carrion entomofauna during the second and third year of decomposition. Our results confirmed previous observations from central Europe that several Coleoptera species are able to recolonize cadavers during the second and third year after death, with their larvae residing on the remains for long periods. The season of cadaver exposure appears to be a main factor determining the composition of the insect fauna that exploits the remains during subsequent years. Our results suggest that it might be possible to estimate the year and season of death analyzing the composition of the insect fauna occurring on a cadaver. The present data highlight the need for further studies on the long-term insect succession on cadavers in different seasons and geographical areas, in order to fully understand its patterns and ensure its proper application in PMI estimations.
Keywords: Coleoptera; Diptera; Forensic entomology; Insect succession; Post-mortem interval; Recolonization.