Detection and quantification of carbendazim in Oolong tea by surface-enhanced Raman spectroscopy and gold nanoparticle substrates

Xi Chen; Mengshi Lin; Lin Sun; Taotao Xu; Keqiang Lai; Meizhen Huang; Hetong Lin

doi:10.1016/j.foodchem.2019.04.085

Detection and quantification of carbendazim in Oolong tea by surface-enhanced Raman spectroscopy and gold nanoparticle substrates

Food Chem. 2019 Sep 30:293:271-277. doi: 10.1016/j.foodchem.2019.04.085. Epub 2019 Apr 25.

Authors

Xi Chen¹, Mengshi Lin², Lin Sun³, Taotao Xu⁴, Keqiang Lai⁴, Meizhen Huang⁵, Hetong Lin⁶

Affiliations

¹ College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
² Food Science Program, Division of Food Systems & Bioengineering, University of Missouri, Columbia, MO 65211-5160, USA. Electronic address: linme@missouri.edu.
³ Food Science Program, Division of Food Systems & Bioengineering, University of Missouri, Columbia, MO 65211-5160, USA.
⁴ College of Food Science and Technology, Shanghai Ocean University, No. 999 Hucheng Huan Road, Lingang New City, Shanghai 201306, China.
⁵ Department of Instrument Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
⁶ College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China. Electronic address: hetonglin@163.com.

PMID: 31151611
DOI: 10.1016/j.foodchem.2019.04.085

Abstract

The contamination of pesticide residues in Oolong tea has raised much concern in recent years. The objective of this study was to synthesize gold nanoparticles (AuNPs) and develop surface-enhanced Raman spectroscopy (SERS) methods for detection and quantification of pesticides in Oolong tea. Facile synthesis of spherical and monodispersed AuNPs with an average diameter of 15 nm was achieved, which induced strong electromagnetic fields in SERS analysis. AuNP substrates were employed for rapid detection and quantification of carbendazim in Tieguanyin Oolong tea. Partial least squares (PLS) analysis and leave-one-out cross validation were utilized in spectral data analysis. The PLS results for Oolong tea samples were obtained: R value = 0.964; the detection limit = 100 μg/kg. These results demonstrate that SERS coupled with gold nanoparticle substrate is a simple, rapid, and sensitive analytical tool for measurement and quantification of carbendazim residues in Oolong tea.

Keywords: Carbendazim; Gold nanoparticles; Oolong tea; Partial least squares (PLS); Pesticides; Surface-enhanced Raman spectroscopy (SERS).

MeSH terms

Benzimidazoles / analysis*
Carbamates / analysis*
Color
Gold / chemistry*
Limit of Detection
Metal Nanoparticles / chemistry*
Pesticide Residues / analysis*
Pesticides / analysis*
Spectrum Analysis, Raman / methods*
Tea / chemistry*

Substances

Benzimidazoles
Carbamates
Pesticide Residues
Pesticides
Tea
Gold
carbendazim