Pharmacological Tools for the Study of H2S Contribution to Angiogenesis

Lucia Morbidelli; Martina Monti; Erika Terzuoli

doi:10.1007/978-1-4939-9528-8_11

Pharmacological Tools for the Study of H₂S Contribution to Angiogenesis

Methods Mol Biol. 2019:2007:151-166. doi: 10.1007/978-1-4939-9528-8_11.

Authors

Lucia Morbidelli¹, Martina Monti², Erika Terzuoli²

Affiliations

¹ Department of Life Sciences, University of Siena, Siena, Italy. lucia.morbidelli@unisi.it.
² Department of Life Sciences, University of Siena, Siena, Italy.

PMID: 31148112
DOI: 10.1007/978-1-4939-9528-8_11

Abstract

Recently, hydrogen sulfide (H₂S) has been characterized as an endogenous mediator able to control a series of cellular and tissue functions relevant for tissue homeostasis and repair such as angiogenesis. This chapter describes the tools and their use in a set of angiogenesis assays performed by using cultured endothelial cells in order to study the relevance of exogenous or endogenous H₂S production and release during the occurrence of angiogenesis.

Keywords: Angiogenesis; Chemoinvasion; Cystathionine γ-lyase; Endothelial cells; Gelatinase; Hydrogen sulfide; Migration; Permeability; Proliferation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Human Umbilical Vein Endothelial Cells / cytology
Human Umbilical Vein Endothelial Cells / metabolism*
Humans
Hydrogen Sulfide / metabolism*
Hydrogen Sulfide / pharmacology*
Neovascularization, Physiologic / drug effects*

Substances

Hydrogen Sulfide