Measurement of 4-Hydroxynonenal (4-HNE) Protein Adducts by ELISA

Kosha Mehta; Vinood B Patel

doi:10.1007/978-1-4939-9463-2_4

Measurement of 4-Hydroxynonenal (4-HNE) Protein Adducts by ELISA

Methods Mol Biol. 2019:1990:43-52. doi: 10.1007/978-1-4939-9463-2_4.

Authors

Kosha Mehta¹, Vinood B Patel²

Affiliations

¹ School of Applied Sciences, London South Bank University, SE1 0AA, London, UK.
² School of Life Sciences, University of Westminster, Westminster, 6UWW1W, UK. v.b.patel@westminster.ac.uk.

PMID: 31148061
DOI: 10.1007/978-1-4939-9463-2_4

Abstract

Enzyme linked immunosorbent assay (ELISA) is a widely used technique for the measurement of antigens and antibodies alike. We describe here procedures, indirect ELISA and sandwich ELISA for the detection of 4-hydroxynonenal protein adducts. These adducts are stable compounds formed within cells and bodily fluids under conditions of oxidative stress. They can act as sensitive biomarkers of oxidative stress and are directly linked to disease pathology.

Keywords: 4-HNE; 4-Hydroxynonenal; Anti-HNE antibodies; Dot blot; ELISA; HRP-labeled antibodies; Indirect ELISA; Sandwich ELISA.

MeSH terms

Aldehydes / analysis
Aldehydes / metabolism*
Antibodies / metabolism*
Enzyme-Linked Immunosorbent Assay / methods*
Humans
Proteins / analysis
Proteins / metabolism*

Substances

Aldehydes
Antibodies
Proteins
4-hydroxy-2-nonenal