Porphyrin‑silver nanoparticles hybrids: Synthesis, characterization and antibacterial activity

R Elashnikov; M Radocha; I Panov; S Rimpelova; P Ulbrich; A Michalcova; V Svorcik; O Lyutakov

doi:10.1016/j.msec.2019.04.029

Porphyrin‑silver nanoparticles hybrids: Synthesis, characterization and antibacterial activity

Mater Sci Eng C Mater Biol Appl. 2019 Sep:102:192-199. doi: 10.1016/j.msec.2019.04.029. Epub 2019 Apr 14.

Authors

R Elashnikov¹, M Radocha¹, I Panov², S Rimpelova³, P Ulbrich³, A Michalcova⁴, V Svorcik¹, O Lyutakov⁵

Affiliations

¹ Department of Solid State Engineering, University of Chemistry and Technology, Prague, Czech Republic.
² Institute of Chemical Process Fundamentals of the CAS, v.v.i., Prague, Czech Republic.
³ Department of Biochemistry and Microbiology, University of Chemistry and Technologye, Prague, Czech Republic.
⁴ Department of Metals and Corrosion Engineering, University of Chemistry and Technology, Prague, Czech Republic.
⁵ Department of Solid State Engineering, University of Chemistry and Technology, Prague, Czech Republic. Electronic address: lyutakoo@vscht.cz.

PMID: 31146990
DOI: 10.1016/j.msec.2019.04.029

Abstract

The preparation of light-activated hybrid antibacterial agent combining the porphyrin molecules, bound to the silver nanoparticles (AgNPs) surface is reported. AgNPs were synthesized by N-methyl-2-pyrrolidone-initiated reduction without additional reducing agents. The chemical structure of protoporphyrin IX was modified with the aim to introduce thiol groups. The size distribution and shape features of AgNPs were checked using TEM and HRTEM microscopies. The introduction of thiol groups into the porphyrin was proved by IR spectroscopy. The AgNPs-porphyrin binding was performed in solution and confirmed by fluorescence quenching, Raman spectroscopy and energy-filtered transmission electron microscopy (EFTEM). The antibacterial tests were performed against S. epidermidis and E. coli upon to LED illumination and in the dark. The synergetic effect of AgNPs and porphyrin as well as light activation of the created antibacterial conjugates were observed.

Keywords: Antibacterial; Light-triggered; Porphyrin; Silver nanoparticles.

MeSH terms

Anti-Bacterial Agents / chemical synthesis*
Anti-Bacterial Agents / pharmacology*
Escherichia coli / drug effects
Luminescence
Metal Nanoparticles / chemistry*
Metal Nanoparticles / ultrastructure
Microbial Sensitivity Tests
Porphyrins / chemical synthesis*
Porphyrins / chemistry
Porphyrins / pharmacology*
Silver / chemistry
Silver / pharmacology*
Staphylococcus epidermidis / drug effects

Substances

Anti-Bacterial Agents
Porphyrins
Silver