Abstract
Osteoarthritis (OA) is a common joint disease for which a safe and reliable treatment has yet to be developed. Here, we demonstrated the potential benefit of treatment with paeonol, a derivative of Paeonia suffruticosa, in the treatment and prevention of OA. Chondrogenic cell line ATDC5 cells were cultured with IL-1β and the effects of paeonol were assessed through qRT-PCR, western blot analysis, MTT, ELISA, and NF-κB luciferase reporter gene assay. Our findings demonstrate a novel ability of paeonol to inhibit numerous factors of OA, including expressions of IL-6, TNF-α, NOX2, PTGS2, NUCB2/nesfatin-1, ICAM-1, VCAM-1, MMP-3/13, degradation of type II collagen, and NF-κB activation through the rescue of IκBα. Additionally, we assessed the effects of paeonol on cell viability to confirm its safety. These findings implicate a valuable potential role of paeonol in the treatment and prevention of OA.
Keywords:
NADPH oxidase 2 (NOX2); Osteoarthritis (OA); interleukin-1β (IL-1β); matrix metalloproteinases (MMPs); nuclear factor-κB (NF-κB); paeonol; type II collagen.
MeSH terms
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Acetophenones / pharmacology*
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Acetophenones / therapeutic use
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Calcium-Binding Proteins / metabolism
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Cell Line
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Cell Survival / drug effects
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Chondrocytes / drug effects*
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Chondrocytes / metabolism
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Chondrocytes / pathology
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Collagen Type II / metabolism*
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Cyclooxygenase 2 / metabolism
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DNA-Binding Proteins / metabolism
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Gene Expression Regulation, Enzymologic / drug effects
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Humans
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Intercellular Adhesion Molecule-1 / metabolism
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Interleukin-1beta / pharmacology*
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Matrix Metalloproteinase 13 / metabolism
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Matrix Metalloproteinase 3 / metabolism
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NADPH Oxidase 2 / metabolism
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NF-KappaB Inhibitor alpha / metabolism
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NF-kappa B / metabolism
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Nerve Tissue Proteins / metabolism
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Nucleobindins
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Osteoarthritis / drug therapy
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Osteoarthritis / metabolism*
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Osteoarthritis / pathology*
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Phosphorylation / drug effects
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Proteolysis / drug effects*
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Signal Transduction / drug effects
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Vascular Cell Adhesion Molecule-1 / metabolism
Substances
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Acetophenones
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Calcium-Binding Proteins
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Collagen Type II
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DNA-Binding Proteins
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Interleukin-1beta
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NF-kappa B
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NUCB2 protein, human
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Nerve Tissue Proteins
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Nucleobindins
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Vascular Cell Adhesion Molecule-1
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Intercellular Adhesion Molecule-1
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NF-KappaB Inhibitor alpha
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paeonol
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Cyclooxygenase 2
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NADPH Oxidase 2
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Matrix Metalloproteinase 13
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Matrix Metalloproteinase 3