Antiviral activity of brequinar against foot-and-mouth disease virus infection in vitro and in vivo

Shi-Fang Li; Mei-Jiao Gong; Yue-Feng Sun; Jun-Jun Shao; Yong-Guang Zhang; Hui-Yun Chang

doi:10.1016/j.biopha.2019.108982

Antiviral activity of brequinar against foot-and-mouth disease virus infection in vitro and in vivo

Biomed Pharmacother. 2019 Aug:116:108982. doi: 10.1016/j.biopha.2019.108982. Epub 2019 May 27.

Authors

Shi-Fang Li¹, Mei-Jiao Gong¹, Yue-Feng Sun², Jun-Jun Shao², Yong-Guang Zhang², Hui-Yun Chang³

Affiliations

¹ State Key Laboratory of Veterinary Etiological Biology, OIE/National Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, Gansu, China.
² State Key Laboratory of Veterinary Etiological Biology, OIE/National Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, Gansu, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu 225009, China.
³ State Key Laboratory of Veterinary Etiological Biology, OIE/National Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, Gansu, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu 225009, China. Electronic address: changhuiyun@caas.cn.

PMID: 31146110
DOI: 10.1016/j.biopha.2019.108982

Abstract

Foot-and-mouth disease (FMD) is one of the most highly contagious animal disease that affects cloven-hoofed animals. However, the FMD vaccine does not provide effective protection until adaptive immune protection elicited by the vaccination occurs. Therefore, an alternative application of antiviral agents for inhibition of the FMD virus (FMDV) is needed. Here, we demonstrated that brequinar could exhibit antiviral activity in swine kidney cells (IBRS-2 cells) infected with two different FMDV serotypes. Subsequently, in vivo activity of brequinar was confirmed in a mouse model of infection. Specifically, brequinar at a concentration of 50 μg, provided 25% protection for 5 days following FMDV challenge. These results suggested that brequinar could be used as effective antiviral agent against FMD.

Keywords: Antiviral activity; Brequinar; Foot and mouth disease virus; Suckling mouse; in vivo.

MeSH terms

Animals
Antiviral Agents / chemistry
Antiviral Agents / pharmacology
Antiviral Agents / therapeutic use*
Biphenyl Compounds / chemistry
Biphenyl Compounds / pharmacology
Biphenyl Compounds / therapeutic use*
Cell Death / drug effects
Cell Line
Foot-and-Mouth Disease / drug therapy*
Foot-and-Mouth Disease / virology*
Foot-and-Mouth Disease Virus / drug effects
Foot-and-Mouth Disease Virus / physiology*
Myocardium / pathology
Swine
Uridine / pharmacology

Substances

Antiviral Agents
Biphenyl Compounds
brequinar
Uridine