An unusually high production of hepatic aflatoxin B1-dihydrodiol, the possible explanation for the high susceptibility of ducks to aflatoxin B1

Abstract

A study was conducted to determine the enzymatic kinetic parameters V_max, K_M, and intrinsic clearance (CL_int) for the hepatic in vitro production of aflatoxin B₁-dihydrodiol (AFB₁-dhd) from aflatoxin B₁ (AFB₁) in four commercial poultry species, ranging in sensitivity to AFB₁ from highest (ducks) to lowest (chickens). Significant but small differences were seen for V_max, while large significant differences were observed for K_M. However, the largest inter-species differences were observed for the CL_int parameter, with ducks being extraordinarily efficient in converting AFB₁ into AFB₁-dhd. Since AFB₁-dhd is considered the metabolite responsible for the acute toxic effects of AFB₁, the high hepatic production of AFB₁-dhd from AFB₁ in ducks is the possible biochemical explanation for the extraordinary high sensitivity of this poultry species to the adverse effects of AFB₁.