Development and optimization of an immunoassay for the detection of Hg(II) in lake water

Xuexue Jin; Rong He; Xingrong Ju; Jie Zhang; Mingjie Wang; Changrui Xing; Jian Yuan

doi:10.1002/fsn3.991

Development and optimization of an immunoassay for the detection of Hg(II) in lake water

Food Sci Nutr. 2019 Apr 6;7(5):1615-1622. doi: 10.1002/fsn3.991. eCollection 2019 May.

Authors

Xuexue Jin¹, Rong He¹, Xingrong Ju¹, Jie Zhang², Mingjie Wang¹, Changrui Xing¹, Jian Yuan¹

Affiliations

¹ College of Food Science and Engineering/Collaborative Innovation Center for Modern Grain Circulation and Safety/Key Laboratory of Grains and Oils Quality Control and Processing Nanjing University of Finance and Economics Nanjing China.
² School of Grain Science and Technology JiangSu University of Science and Technology Zhenjiang China.

Abstract

In this paper, an indirect competitive enzyme-linked immunosorbent assay (IC-ELISA) has been developed and optimized to detect Hg(II) in tap water and lake water based on a monoclonal antibody (mAb-A24). Some stabilizing additives (Gelatin, bovine serum albumin [BSA], polyvinyl alcohol [PVA], and polyvinyl pyrrolidone [PVP]) and surfactant (Tween-20) have been investigated thoroughly in the optimization process. Under the optimal condition, the 50% half maximal inhibitory concentration (IC₅₀) and limit of detection (LOD) were 1.68 and 0.079 ng/ml, respectively. These anti-Hg mAbs also have some affinity with methyl mercury (CH₃Hg) and with no cross-reactivity with other thirteen metal ions. The developed method has shown satisfactory recovery of Hg(II), ranged between 91% and 116%, from tap water and lake water. Therefore, this immunoassay can be used to detect trace Hg(II) in environment water.

Keywords: Hg(II); antibody; immunoassay; lake water; tap water.