Julichrome derivatives and gliotoxin from a soil derived Streptomyces sp

Abstract

Six julichrome derivatives including a new monomeric julichrome named as julichrome Q₁₀ (1), and previous reported julichrome Q₆ (2), julichrome Q_6.6 (4), julichrome Q_3.5 (5), julichrome Q_5.6 (6), julichrome Q_2.3 (7), along with a diketopiperazine gliotoxin (3) were isolated from a soil derived strain Streptomyces sp. The structures of these compounds were identified by HR-ESI-MS, UV, IR and NMR methods. The isolated compounds were tested for their in vitro cytotoxicity against human hepatocarcinoma HepG-2 and SMMC-7721 cell lines, human breast cancer MCF-7 and MDA-MB-231 cell lines, and human normal heptical LO2 cell line. Gliotoxin (3) showed the most cytotoxic activity against the tested tumor cell lines, with IC₅₀ values ranging from 0.11 to 1.45 μM. Julichrome Q_6.6 (4) displayed selective cytotoxic activity against SMMC-7721, MCF-7 and MDA-MB-231 cell lines.

Keywords: Streptomyces; cytotoxic activity; gliotoxin; julichrome.