DNA-Encoded Macrocyclic Peptide Library

Methods Mol Biol. 2019:2001:273-284. doi: 10.1007/978-1-4939-9504-2_12.

Abstract

DNA-encoded library technology (ELT) is a cutting-edge enabling technology platform for drug discovery. Here we describe how to design and synthesize a macrocyclic DNA-encoded library; how to perform selection, sequencing, and data analysis to identify potential active peptides; and how to synthesize off-DNA peptides to confirm activity. This approach provides an effective tool for pharmaceutical research based on peptides.

Keywords: Affinity selection; DNA-encoded library; Macrocyclic peptides.

MeSH terms

  • DNA / chemistry*
  • Drug Discovery
  • High-Throughput Nucleotide Sequencing
  • Macrocyclic Compounds / chemical synthesis*
  • Macrocyclic Compounds / chemistry
  • Peptide Library*
  • Peptides, Cyclic / chemical synthesis
  • Peptides, Cyclic / chemistry*
  • Protein Interaction Domains and Motifs
  • Small Molecule Libraries / metabolism

Substances

  • Macrocyclic Compounds
  • Peptide Library
  • Peptides, Cyclic
  • Small Molecule Libraries
  • DNA