Liquid biphasic flotation for the purification of C-phycocyanin from Spirulina platensis microalga

Kit Wayne Chew; Shir Reen Chia; Rambabu Krishnamoorthy; Yang Tao; Dinh-Toi Chu; Pau Loke Show

doi:10.1016/j.biortech.2019.121519

Liquid biphasic flotation for the purification of C-phycocyanin from Spirulina platensis microalga

Bioresour Technol. 2019 Sep:288:121519. doi: 10.1016/j.biortech.2019.121519. Epub 2019 May 20.

Authors

Kit Wayne Chew¹, Shir Reen Chia¹, Rambabu Krishnamoorthy², Yang Tao³, Dinh-Toi Chu⁴, Pau Loke Show⁵

Affiliations

¹ Department of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, 43500 Semenyih, Selangor, Malaysia.
² Department of Chemical Engineering, School of Chemical Engineering, Vellore Institute of Technology, Vellore 632014, India.
³ College of Food Science and Technology, Nanjing Agricultural University, 1 Weigang Road, Nanjing, Jiangsu 210095, China.
⁴ Faculty of Biology, Hanoi National University of Education, 136 Xuan Thuy, Cau Giay, Hanoi, Viet Nam; Institute for Research and Development, Duy Tan University, Danang, Viet Nam; School of Odonto Stomatology, Hanoi Medical University, Hanoi, Viet Nam.
⁵ Department of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, 43500 Semenyih, Selangor, Malaysia. Electronic address: PauLoke.Show@nottingham.edu.my.

PMID: 31128541
DOI: 10.1016/j.biortech.2019.121519

Abstract

Liquid biphasic flotation (LBF), an integrated process of liquid biphasic system (LBS) and adsorptive bubbles flotation, was used for the purification of C-phycocyanin from S. platensis microalgae. Various experimental parameters such as type of phase forming polymer and salt, concentration of phase forming components, system pH, volume ratio, air flotation time and crude extract concentration were evaluated to maximise the C-phycocyanin recovery yield and purity. The optimal conditions for the LBF system achieving C-phycocyanin purification fold of 3.49 compared to 2.43 from the initial LBF conditions was in polyethylene glycol (PEG) 4000 and potassium phosphate combination, with 250 g/L of polymer and salt concentration each, volume ratio of 1:0.85, system pH of 7.0, air flotation duration of 7 min and phycocyanin crude extract concentration of 0.625 %w/w. The LBF has effectively enhanced the purification of C-phycocyanin in a cost effective and simple processing.

Keywords: C-phycocyanin; Liquid biphasic flotation; Pigment; Purification; Spirulina platensis.

MeSH terms

Adsorption
Microalgae*
Phycocyanin
Spirulina*

Substances

Phycocyanin