Chromatin Immunoprecipitation (ChIP) of Plasmid-Bound Proteins in Xenopus Egg Extracts

Methods Mol Biol. 2019:1999:173-184. doi: 10.1007/978-1-4939-9500-4_10.

Abstract

Xenopus egg extracts provide a cell-free system to analyze various aspects of chromatin biology. Here we describe a modified method of chromatin immunoprecipitation (ChIP) to detect the interaction of proteins with plasmid DNA incubated in extract. The combination of ChIP and Xenopus egg extracts provides a highly versatile and tractable approach to analyze dynamic protein-DNA interactions with great spatial and temporal detail.

Keywords: Cell-free system; Chromatin immunoprecipitation (ChIP); DNA repair; Nucleoplasmic extract; Xenopus egg extract.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Cell-Free System
  • Chromatin / genetics
  • Chromatin / metabolism*
  • Chromatin Immunoprecipitation / methods*
  • DNA / genetics*
  • DNA / metabolism
  • Oocytes
  • Plasmids / genetics
  • Protein Binding / genetics
  • Spatio-Temporal Analysis
  • Xenopus Proteins / genetics
  • Xenopus Proteins / metabolism*
  • Xenopus laevis

Substances

  • Chromatin
  • Xenopus Proteins
  • DNA