The purpose of this research was to evaluate the influence of the structural components of different 3rd generation artificial turf football field systems on the biomechanical response of impact attenuation in amateur football players. A total of 12 amateur football players (24.3 ± 3.7 years, 73.5 ± 5.5 kg, 178.3 ± 4.1 cm and 13.7 ± 4.3 years of sport experience) were evaluated on three third generation artificial turf systems (ATS) with different structural components. ATS were composed of asphalt sub-base and 45 mm of fibre height with (ATS1) and without (ATS2) elastic layer or compacted granular sub-base, 60 mm of fibre height without elastic layer (ATS3). Two triaxial accelerometers were firmly taped to the forehead and the distal end of the right tibia of each individual. The results reveal a higher force reduction on ATS3 in comparison to ATS1 (+6.24%, CI95%: 1.67 to 10.92, ES: 1.07; p < 0.05) and ATS2 (+21.08%, CI95%: 16.51 to 25.66, ES: 2.98; p < 0.05) elastic layer. Tibia acceleration rate was lower on ATS3 than ATS1 (-0.32, CI95%: -0.60 to -0.03, ES: 4.23; p < 0.05) and ATS2 (-0.35, CI95%: -0.64 to -0.06; ES: 4.69; p < 0.05) at 3.3 m/s. A very large correlation (r = 0.7 to 0.9; p < 0.05) was found between energy restitution and fibre height in both head and tibial peak acceleration and stride time. In conclusion, structural components (fibre height, infill, sub-base and elastic layer) determine the mechanical properties of artificial turf fields. A higher force reduction and lower energy restitution diminished the impact received by the player which could protect against injuries associated with impacts compared to harder artificial turf surfaces.