New discrimination tools for harvest year and varieties of white wines based on hydrophilic interaction liquid chromatography with amperometric detection

Clara Pérez-Ràfols; Xavier Subirats; Núria Serrano; José Manuel Díaz-Cruz

doi:10.1016/j.talanta.2019.03.099

New discrimination tools for harvest year and varieties of white wines based on hydrophilic interaction liquid chromatography with amperometric detection

Talanta. 2019 Aug 15:201:104-110. doi: 10.1016/j.talanta.2019.03.099. Epub 2019 Apr 2.

Authors

Clara Pérez-Ràfols¹, Xavier Subirats¹, Núria Serrano², José Manuel Díaz-Cruz¹

Affiliations

¹ Department of Chemical Engineering and Analytical Chemistry, Universitat de Barcelona, Martí i Franquès 1-11, E-08028, Barcelona, Spain.
² Department of Chemical Engineering and Analytical Chemistry, Universitat de Barcelona, Martí i Franquès 1-11, E-08028, Barcelona, Spain. Electronic address: nuria.serrano@ub.edu.

PMID: 31122399
DOI: 10.1016/j.talanta.2019.03.099

Abstract

A simple HPLC-EC method based on hydrophilic interaction liquid chromatography with amperometric detection through gold screen-printed electrodes has been developed and applied for the first time to the determination of aminothiols in white wines. Moreover, the coupling of the method with partial least squares discriminant analysis (PLS-DA) using the analysed aminothiols as biomarkers provides wine discrimination in terms of harvest year. White wine samples were directly injected and chromatographic areas, together with pH and redox potential values, allowed a successful discrimination of wines from different harvest years with a global classification rate of 97.8%. The developed HPLC-EC method also generated characteristic fingerprints that were combined with PLS-DA to classify wines according to three wine varieties, with a global classification rate of 95.3%.

Keywords: Aminothiols; Electrochemical detection; Gold screen-printed electrode; HILIC; Partial least squares discriminant analysis; Wine analysis.