Human induced pluripotent stem cell line HMUi001-A derived from corneal stromal cells

Timur Bikkuzin; Yan Shi; Baoqi Sun; Yiyuan Guo; Xin Jin; Zhenbo Han; Valentin Pavlov; Hong Zhang

doi:10.1016/j.scr.2019.101409

Human induced pluripotent stem cell line HMUi001-A derived from corneal stromal cells

Stem Cell Res. 2019 May:37:101409. doi: 10.1016/j.scr.2019.101409. Epub 2019 Feb 15.

Authors

Timur Bikkuzin¹, Yan Shi², Baoqi Sun², Yiyuan Guo², Xin Jin², Zhenbo Han³, Valentin Pavlov⁴, Hong Zhang⁵

Affiliations

¹ Eye Hospital, First Affiliated Hospital of Harbin Medical University, Harbin, China; Central Laboratory of Scientific Research, Bashkir State Medical University, Ufa, Russian Federation.
² Eye Hospital, First Affiliated Hospital of Harbin Medical University, Harbin, China.
³ Department of Pharmacy, the Second Affiliated Hospital of Harbin Medical University (Institute of Clinical Pharmacy, the Heilongjiang Key Laboratory of Drug Research, Harbin Medical University), Harbin, China; Department of Pharmacology (the State-Province Key Laboratories of Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education), College of Pharmacy, Harbin Medical University, Harbin, China.
⁴ Central Laboratory of Scientific Research, Bashkir State Medical University, Ufa, Russian Federation.
⁵ Eye Hospital, First Affiliated Hospital of Harbin Medical University, Harbin, China. Electronic address: zelmykqnwyh@163.com.

PMID: 31121475
DOI: 10.1016/j.scr.2019.101409

Abstract

A human corneal stroma induced pluripotent stem cell (HMUi001-A) line was created from primary cultured human corneal fibroblasts. Reprogramming was performed using episomal vector delivery of OCT4, SOX2, KLF4, L-MYC and LIN28. Further characterization of the HMUi001-A confirmed that the cell line was pluripotent, free from Epstein Barr viral genome, and retained normal karyotype.

MeSH terms

Adult
Cell Differentiation*
Cells, Cultured
Cellular Reprogramming*
Corneal Stroma / cytology*
Fibroblasts / cytology*
Humans
Induced Pluripotent Stem Cells / cytology*
Kruppel-Like Factor 4
Male
Young Adult