Optimization of Resolving Power, Fragmentation, and Mass Calibration in an Orbitrap Spectrometer for Analysis of 24 Pesticide Metabolites in Urine

Pablo Dualde; Clara Coscollà; Agustin Pastor; Vicent Yusà

doi:10.1155/2019/1917369

Optimization of Resolving Power, Fragmentation, and Mass Calibration in an Orbitrap Spectrometer for Analysis of 24 Pesticide Metabolites in Urine

Int J Anal Chem. 2019 Apr 17:2019:1917369. doi: 10.1155/2019/1917369. eCollection 2019.

Authors

Pablo Dualde^{1

2}, Clara Coscollà^{1

3}, Agustin Pastor², Vicent Yusà^{1

2

3}

Affiliations

¹ Foundation for the Promotion of Health and Biomedical Research in the Valencian Region, FISABIO-Public Health, 21 Avenida Catalunya, 46020 Valencia, Spain.
² Analytical Chemistry Department, University of Valencia, Edifici Jeroni Muñoz, Dr. Moliner 50, 46100 Burjassot, Spain.
³ Public Health Laboratory of Valencia, 21 Avenida Catalunya, 46020 Valencia, Spain.

Abstract

Mass spectrometer parameters such as Resolving Power, type of fragmentation, and mass calibration mode were optimized in the analysis of 24 pesticide metabolites in human urine using Ultra-High Pressure Liquid Chromatography coupled to Orbitrap High-Resolution Mass Spectrometer (UHPLC-HRMS). The best results were achieved with a Resolving Power of 25,000 FWHM and by applying Collision Induced Dissociation fragmentation mode (40 eV).