Analysis of Nitrosamines in Processed Meat Products in Medan City by Liquid Chromatography-Mass Spectrometry

Open Access Maced J Med Sci. 2019 Apr 16;7(8):1382-1387. doi: 10.3889/oamjms.2019.261. eCollection 2019 Apr 30.

Abstract

Background: Nitrosamine is a carcinogen and the maximum level in processed meat products set by WHO.

Aim: The purpose of this study was to determine nitrosamine levels in meat products in Medan City and compared nitrosamine levels with standards set by WHO.

Methods: The samples analysed were obtained from Berastagi Supermarket, sausages, burgers, corned beef and smoked beef. Nitrosamine levels were determined by reverse phase liquid chromatography-mass spectrometry.

Results: The results showed that only 5 out of 20 samples of N-nitroso-thiazolidine-4-carboxylic acid (NTCA) nitrosamine ranged from 501.290 to 4227.492 µg/kg. The highest level of NTCA was found in smoked beef (Chiefs), which is 4227.492 µg/kg. N-nitroso-2-methyl-thiazolidine-4-carboxylic acid (NMTCA) was contained in all the samples analysed which ranged from 20.50 to 989.175 µg/kg. The highest NMTCA nitrosamine content was found in smoked beef (Chiefs), which is 989.175 µg/kg.

Conclusion: From this study reveal that nitrosamines in meat products exceed the maximum standards set by WHO (10 µg/kg).

Keywords: LC-MS/MS; Meat products; Nitrosamines.