In this work, two amphiphilic gluconamide-tailored anthracene gelators 1 and 2 have been synthesized, and found to form stable hydrogels with fibril structures. The stimuli-responsive behaviors of hydrogel 1 and 2 were investigated thoroughly by temperature-dependent 1H NMR, UV-Vis, rheometry, transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The results showed that hydrogel 1 exhibited multiple responsive behaviours upon exposure to stimuli including temperature, anions, light, electron-deficient chemicals and external stress; conversely, hydrogel 2 showed a distinct responsive phenomenon attributed to a subtle structural difference in the linker. This work demonstrates that gluconamide-tailored anthracene gelators could be a potential soft material and highlights the importance of a precisely designed structure.