In this paper, the tracking performance limitation of networked control systems (NCSs) is studied. The NCSs are considered as continuous-time linear multi-input multioutput (MIMO) systems with random reference noises. The controlled plants include unstable poles and nonminimum phase (NMP) zeros. The output feedback path is affected by multiple communication constraints. We focus on some basic communication constraints, including additive white noise (AWN), quantization noise, bandwidth, as well as encoder-decoder. The system performance is evaluated with the tracking error energy, and used a two-degree-of-freedom (2DOF) controller. The explicit representation of the tracking performance is given in this paper. The results indicate the tracking performance limitations rely to internal characteristics of the plant (unstable poles and NMP zeros), reference noises [the reference noise power distribution (RNPD) and its directions], and the characteristics of communication constraints. The characteristics of communication constraints include communication noise power distribution (CNPD); quantization noise power distribution (QNPD), and their distribution directions; transform bandwidth allocation (TBA); transform encoder-decoder allocation (TEA), and their allocation directions; and NMP zeros and MP part of bandwidth. Moreover, the tracking performance limitations are also affected by the angles between the each transform NMP zero direction and RNPD direction, and these angles between each transform unstable poles direction and the direction of communication constraint distribution/allocation. In addition, for MIMO NCSs, bandwidth (there are not identical two channels) can always affect the direction of unstable poles, and the channel allocation of bandwidth and encode-decode may be used for a feasible method for the performance allocation of each channel. Finally, an instance is given for verifying the effectiveness of the theoretical outcomes.